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PCR-based antibiotic resistance gene fingerprint patterns of E. coli strain isolated from sample P60 The figure displays amplification results for 92 genetic markers associated with AMR. Each lane represents a specific PCR target. 1: β-lactam TEM group. 2–5: β-lactam CTX-M group. 6–10: β-lactam ACC, DHA, FOX, VEB, SHV groups. 11–12: β-lactam CMY group. 13–19: β-lactam IMP, SMP, VIM, OXA, BIC, KPC, NDM groups. 20–21: chloramphenicol ( cmlA , catA1 ). 22–26: colistin ( mcr -1, mcr -2, mcr -3, mcr -4, mcr -5). 27: florfenicol ( floR ). 28–30: gentamicin ( aac(3)-IV , ant(2″)-I , aac(3)-II ). 31: linezolid ( optrA ). 32–34: neomycin ( aph(3′)-III-I variants). 35–45: quinolones ( gyrA Salmonella , parC Salmonella , gyrA E. coli , parC E. coli , qnrA , qnrB , qnrC , qnrD , qnrS , aac(6′)-Ib-cr , qepA ). 46–49: streptomycin ( strA , strB , aadA2 , aadE ). 50–52: sulfamethoxazole ( sul1 , sul2 , sul3 ). 53–72: tetracyclines ( tetA , tetB , tetC , tetD , tetE , tetG , tetH , tetK , tetL , tetM , tetO , tetS , tetT , tetW , tetZ , tet31 , tet32 , tet33 , tet34 , tet39 ). 73–75: glycopeptides ( vanA , vanB , vanX ). 76–80: macrolides ( ermA , ermB , ermC , ermF ). 81–88: streptogramins ( vat , vatB , vatD , vatE , vgaA , vgaB , vgbA , vgbB ). 89–92: MRSA-associated markers ( spa , mecA , mecC , pvl ). M: <t>DNA</t> ladder.
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PCR-based antibiotic resistance gene fingerprint patterns of E. coli strain isolated from sample P60 The figure displays amplification results for 92 genetic markers associated with AMR. Each lane represents a specific PCR target. 1: β-lactam TEM group. 2–5: β-lactam CTX-M group. 6–10: β-lactam ACC, DHA, FOX, VEB, SHV groups. 11–12: β-lactam CMY group. 13–19: β-lactam IMP, SMP, VIM, OXA, BIC, KPC, NDM groups. 20–21: chloramphenicol ( cmlA , catA1 ). 22–26: colistin ( mcr -1, mcr -2, mcr -3, mcr -4, mcr -5). 27: florfenicol ( floR ). 28–30: gentamicin ( aac(3)-IV , ant(2″)-I , aac(3)-II ). 31: linezolid ( optrA ). 32–34: neomycin ( aph(3′)-III-I variants). 35–45: quinolones ( gyrA Salmonella , parC Salmonella , gyrA E. coli , parC E. coli , qnrA , qnrB , qnrC , qnrD , qnrS , aac(6′)-Ib-cr , qepA ). 46–49: streptomycin ( strA , strB , aadA2 , aadE ). 50–52: sulfamethoxazole ( sul1 , sul2 , sul3 ). 53–72: tetracyclines ( tetA , tetB , tetC , tetD , tetE , tetG , tetH , tetK , tetL , tetM , tetO , tetS , tetT , tetW , tetZ , tet31 , tet32 , tet33 , tet34 , tet39 ). 73–75: glycopeptides ( vanA , vanB , vanX ). 76–80: macrolides ( ermA , ermB , ermC , ermF ). 81–88: streptogramins ( vat , vatB , vatD , vatE , vgaA , vgaB , vgbA , vgbB ). 89–92: MRSA-associated markers ( spa , mecA , mecC , pvl ). M: <t>DNA</t> ladder.
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PCR-based antibiotic resistance gene fingerprint patterns of E. coli strain isolated from sample P60 The figure displays amplification results for 92 genetic markers associated with AMR. Each lane represents a specific PCR target. 1: β-lactam TEM group. 2–5: β-lactam CTX-M group. 6–10: β-lactam ACC, DHA, FOX, VEB, SHV groups. 11–12: β-lactam CMY group. 13–19: β-lactam IMP, SMP, VIM, OXA, BIC, KPC, NDM groups. 20–21: chloramphenicol ( cmlA , catA1 ). 22–26: colistin ( mcr -1, mcr -2, mcr -3, mcr -4, mcr -5). 27: florfenicol ( floR ). 28–30: gentamicin ( aac(3)-IV , ant(2″)-I , aac(3)-II ). 31: linezolid ( optrA ). 32–34: neomycin ( aph(3′)-III-I variants). 35–45: quinolones ( gyrA Salmonella , parC Salmonella , gyrA E. coli , parC E. coli , qnrA , qnrB , qnrC , qnrD , qnrS , aac(6′)-Ib-cr , qepA ). 46–49: streptomycin ( strA , strB , aadA2 , aadE ). 50–52: sulfamethoxazole ( sul1 , sul2 , sul3 ). 53–72: tetracyclines ( tetA , tetB , tetC , tetD , tetE , tetG , tetH , tetK , tetL , tetM , tetO , tetS , tetT , tetW , tetZ , tet31 , tet32 , tet33 , tet34 , tet39 ). 73–75: glycopeptides ( vanA , vanB , vanX ). 76–80: macrolides ( ermA , ermB , ermC , ermF ). 81–88: streptogramins ( vat , vatB , vatD , vatE , vgaA , vgaB , vgbA , vgbB ). 89–92: MRSA-associated markers ( spa , mecA , mecC , pvl ). M: <t>DNA</t> ladder.
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PCR-based antibiotic resistance gene fingerprint patterns of E. coli strain isolated from sample P60 The figure displays amplification results for 92 genetic markers associated with AMR. Each lane represents a specific PCR target. 1: β-lactam TEM group. 2–5: β-lactam CTX-M group. 6–10: β-lactam ACC, DHA, FOX, VEB, SHV groups. 11–12: β-lactam CMY group. 13–19: β-lactam IMP, SMP, VIM, OXA, BIC, KPC, NDM groups. 20–21: chloramphenicol ( cmlA , catA1 ). 22–26: colistin ( mcr -1, mcr -2, mcr -3, mcr -4, mcr -5). 27: florfenicol ( floR ). 28–30: gentamicin ( aac(3)-IV , ant(2″)-I , aac(3)-II ). 31: linezolid ( optrA ). 32–34: neomycin ( aph(3′)-III-I variants). 35–45: quinolones ( gyrA Salmonella , parC Salmonella , gyrA E. coli , parC E. coli , qnrA , qnrB , qnrC , qnrD , qnrS , aac(6′)-Ib-cr , qepA ). 46–49: streptomycin ( strA , strB , aadA2 , aadE ). 50–52: sulfamethoxazole ( sul1 , sul2 , sul3 ). 53–72: tetracyclines ( tetA , tetB , tetC , tetD , tetE , tetG , tetH , tetK , tetL , tetM , tetO , tetS , tetT , tetW , tetZ , tet31 , tet32 , tet33 , tet34 , tet39 ). 73–75: glycopeptides ( vanA , vanB , vanX ). 76–80: macrolides ( ermA , ermB , ermC , ermF ). 81–88: streptogramins ( vat , vatB , vatD , vatE , vgaA , vgaB , vgbA , vgbB ). 89–92: MRSA-associated markers ( spa , mecA , mecC , pvl ). M: <t>DNA</t> ladder.
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PCR-based antibiotic resistance gene fingerprint patterns of E. coli strain isolated from sample P60 The figure displays amplification results for 92 genetic markers associated with AMR. Each lane represents a specific PCR target. 1: β-lactam TEM group. 2–5: β-lactam CTX-M group. 6–10: β-lactam ACC, DHA, FOX, VEB, SHV groups. 11–12: β-lactam CMY group. 13–19: β-lactam IMP, SMP, VIM, OXA, BIC, KPC, NDM groups. 20–21: chloramphenicol ( cmlA , catA1 ). 22–26: colistin ( mcr -1, mcr -2, mcr -3, mcr -4, mcr -5). 27: florfenicol ( floR ). 28–30: gentamicin ( aac(3)-IV , ant(2″)-I , aac(3)-II ). 31: linezolid ( optrA ). 32–34: neomycin ( aph(3′)-III-I variants). 35–45: quinolones ( gyrA Salmonella , parC Salmonella , gyrA E. coli , parC E. coli , qnrA , qnrB , qnrC , qnrD , qnrS , aac(6′)-Ib-cr , qepA ). 46–49: streptomycin ( strA , strB , aadA2 , aadE ). 50–52: sulfamethoxazole ( sul1 , sul2 , sul3 ). 53–72: tetracyclines ( tetA , tetB , tetC , tetD , tetE , tetG , tetH , tetK , tetL , tetM , tetO , tetS , tetT , tetW , tetZ , tet31 , tet32 , tet33 , tet34 , tet39 ). 73–75: glycopeptides ( vanA , vanB , vanX ). 76–80: macrolides ( ermA , ermB , ermC , ermF ). 81–88: streptogramins ( vat , vatB , vatD , vatE , vgaA , vgaB , vgbA , vgbB ). 89–92: MRSA-associated markers ( spa , mecA , mecC , pvl ). M: <t>DNA</t> ladder.
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PCR-based antibiotic resistance gene fingerprint patterns of E. coli strain isolated from sample P60 The figure displays amplification results for 92 genetic markers associated with AMR. Each lane represents a specific PCR target. 1: β-lactam TEM group. 2–5: β-lactam CTX-M group. 6–10: β-lactam ACC, DHA, FOX, VEB, SHV groups. 11–12: β-lactam CMY group. 13–19: β-lactam IMP, SMP, VIM, OXA, BIC, KPC, NDM groups. 20–21: chloramphenicol ( cmlA , catA1 ). 22–26: colistin ( mcr -1, mcr -2, mcr -3, mcr -4, mcr -5). 27: florfenicol ( floR ). 28–30: gentamicin ( aac(3)-IV , ant(2″)-I , aac(3)-II ). 31: linezolid ( optrA ). 32–34: neomycin ( aph(3′)-III-I variants). 35–45: quinolones ( gyrA Salmonella , parC Salmonella , gyrA E. coli , parC E. coli , qnrA , qnrB , qnrC , qnrD , qnrS , aac(6′)-Ib-cr , qepA ). 46–49: streptomycin ( strA , strB , aadA2 , aadE ). 50–52: sulfamethoxazole ( sul1 , sul2 , sul3 ). 53–72: tetracyclines ( tetA , tetB , tetC , tetD , tetE , tetG , tetH , tetK , tetL , tetM , tetO , tetS , tetT , tetW , tetZ , tet31 , tet32 , tet33 , tet34 , tet39 ). 73–75: glycopeptides ( vanA , vanB , vanX ). 76–80: macrolides ( ermA , ermB , ermC , ermF ). 81–88: streptogramins ( vat , vatB , vatD , vatE , vgaA , vgaB , vgbA , vgbB ). 89–92: MRSA-associated markers ( spa , mecA , mecC , pvl ). M: <t>DNA</t> ladder.
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PCR-based antibiotic resistance gene fingerprint patterns of E. coli strain isolated from sample P60 The figure displays amplification results for 92 genetic markers associated with AMR. Each lane represents a specific PCR target. 1: β-lactam TEM group. 2–5: β-lactam CTX-M group. 6–10: β-lactam ACC, DHA, FOX, VEB, SHV groups. 11–12: β-lactam CMY group. 13–19: β-lactam IMP, SMP, VIM, OXA, BIC, KPC, NDM groups. 20–21: chloramphenicol ( cmlA , catA1 ). 22–26: colistin ( mcr -1, mcr -2, mcr -3, mcr -4, mcr -5). 27: florfenicol ( floR ). 28–30: gentamicin ( aac(3)-IV , ant(2″)-I , aac(3)-II ). 31: linezolid ( optrA ). 32–34: neomycin ( aph(3′)-III-I variants). 35–45: quinolones ( gyrA Salmonella , parC Salmonella , gyrA E. coli , parC E. coli , qnrA , qnrB , qnrC , qnrD , qnrS , aac(6′)-Ib-cr , qepA ). 46–49: streptomycin ( strA , strB , aadA2 , aadE ). 50–52: sulfamethoxazole ( sul1 , sul2 , sul3 ). 53–72: tetracyclines ( tetA , tetB , tetC , tetD , tetE , tetG , tetH , tetK , tetL , tetM , tetO , tetS , tetT , tetW , tetZ , tet31 , tet32 , tet33 , tet34 , tet39 ). 73–75: glycopeptides ( vanA , vanB , vanX ). 76–80: macrolides ( ermA , ermB , ermC , ermF ). 81–88: streptogramins ( vat , vatB , vatD , vatE , vgaA , vgaB , vgbA , vgbB ). 89–92: MRSA-associated markers ( spa , mecA , mecC , pvl ). M: <t>DNA</t> ladder.
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PCR-based antibiotic resistance gene fingerprint patterns of E. coli strain isolated from sample P60 The figure displays amplification results for 92 genetic markers associated with AMR. Each lane represents a specific PCR target. 1: β-lactam TEM group. 2–5: β-lactam CTX-M group. 6–10: β-lactam ACC, DHA, FOX, VEB, SHV groups. 11–12: β-lactam CMY group. 13–19: β-lactam IMP, SMP, VIM, OXA, BIC, KPC, NDM groups. 20–21: chloramphenicol ( cmlA , catA1 ). 22–26: colistin ( mcr -1, mcr -2, mcr -3, mcr -4, mcr -5). 27: florfenicol ( floR ). 28–30: gentamicin ( aac(3)-IV , ant(2″)-I , aac(3)-II ). 31: linezolid ( optrA ). 32–34: neomycin ( aph(3′)-III-I variants). 35–45: quinolones ( gyrA Salmonella , parC Salmonella , gyrA E. coli , parC E. coli , qnrA , qnrB , qnrC , qnrD , qnrS , aac(6′)-Ib-cr , qepA ). 46–49: streptomycin ( strA , strB , aadA2 , aadE ). 50–52: sulfamethoxazole ( sul1 , sul2 , sul3 ). 53–72: tetracyclines ( tetA , tetB , tetC , tetD , tetE , tetG , tetH , tetK , tetL , tetM , tetO , tetS , tetT , tetW , tetZ , tet31 , tet32 , tet33 , tet34 , tet39 ). 73–75: glycopeptides ( vanA , vanB , vanX ). 76–80: macrolides ( ermA , ermB , ermC , ermF ). 81–88: streptogramins ( vat , vatB , vatD , vatE , vgaA , vgaB , vgbA , vgbB ). 89–92: MRSA-associated markers ( spa , mecA , mecC , pvl ). M: <t>DNA</t> ladder.
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PCR-based antibiotic resistance gene fingerprint patterns of E. coli strain isolated from sample P60 The figure displays amplification results for 92 genetic markers associated with AMR. Each lane represents a specific PCR target. 1: β-lactam TEM group. 2–5: β-lactam CTX-M group. 6–10: β-lactam ACC, DHA, FOX, VEB, SHV groups. 11–12: β-lactam CMY group. 13–19: β-lactam IMP, SMP, VIM, OXA, BIC, KPC, NDM groups. 20–21: chloramphenicol ( cmlA , catA1 ). 22–26: colistin ( mcr -1, mcr -2, mcr -3, mcr -4, mcr -5). 27: florfenicol ( floR ). 28–30: gentamicin ( aac(3)-IV , ant(2″)-I , aac(3)-II ). 31: linezolid ( optrA ). 32–34: neomycin ( aph(3′)-III-I variants). 35–45: quinolones ( gyrA Salmonella , parC Salmonella , gyrA E. coli , parC E. coli , qnrA , qnrB , qnrC , qnrD , qnrS , aac(6′)-Ib-cr , qepA ). 46–49: streptomycin ( strA , strB , aadA2 , aadE ). 50–52: sulfamethoxazole ( sul1 , sul2 , sul3 ). 53–72: tetracyclines ( tetA , tetB , tetC , tetD , tetE , tetG , tetH , tetK , tetL , tetM , tetO , tetS , tetT , tetW , tetZ , tet31 , tet32 , tet33 , tet34 , tet39 ). 73–75: glycopeptides ( vanA , vanB , vanX ). 76–80: macrolides ( ermA , ermB , ermC , ermF ). 81–88: streptogramins ( vat , vatB , vatD , vatE , vgaA , vgaB , vgbA , vgbB ). 89–92: MRSA-associated markers ( spa , mecA , mecC , pvl ). M: DNA ladder.

Journal: iScience

Article Title: Prevalence of ARGs in poultry-associated E. coli in Zhejiang Province, China: A genotypic survey

doi: 10.1016/j.isci.2026.116177

Figure Lengend Snippet: PCR-based antibiotic resistance gene fingerprint patterns of E. coli strain isolated from sample P60 The figure displays amplification results for 92 genetic markers associated with AMR. Each lane represents a specific PCR target. 1: β-lactam TEM group. 2–5: β-lactam CTX-M group. 6–10: β-lactam ACC, DHA, FOX, VEB, SHV groups. 11–12: β-lactam CMY group. 13–19: β-lactam IMP, SMP, VIM, OXA, BIC, KPC, NDM groups. 20–21: chloramphenicol ( cmlA , catA1 ). 22–26: colistin ( mcr -1, mcr -2, mcr -3, mcr -4, mcr -5). 27: florfenicol ( floR ). 28–30: gentamicin ( aac(3)-IV , ant(2″)-I , aac(3)-II ). 31: linezolid ( optrA ). 32–34: neomycin ( aph(3′)-III-I variants). 35–45: quinolones ( gyrA Salmonella , parC Salmonella , gyrA E. coli , parC E. coli , qnrA , qnrB , qnrC , qnrD , qnrS , aac(6′)-Ib-cr , qepA ). 46–49: streptomycin ( strA , strB , aadA2 , aadE ). 50–52: sulfamethoxazole ( sul1 , sul2 , sul3 ). 53–72: tetracyclines ( tetA , tetB , tetC , tetD , tetE , tetG , tetH , tetK , tetL , tetM , tetO , tetS , tetT , tetW , tetZ , tet31 , tet32 , tet33 , tet34 , tet39 ). 73–75: glycopeptides ( vanA , vanB , vanX ). 76–80: macrolides ( ermA , ermB , ermC , ermF ). 81–88: streptogramins ( vat , vatB , vatD , vatE , vgaA , vgaB , vgbA , vgbB ). 89–92: MRSA-associated markers ( spa , mecA , mecC , pvl ). M: DNA ladder.

Article Snippet: DNA Marker (200∼2000 bp) , BBI , Cat#B600339.

Techniques: Isolation, Amplification